Article Dans Une Revue Scientific Reports Année : 2025

Unlocking soybean meal pectin recalcitrance using a multi-enzyme cocktail approach

Lauriane Plouhinec
  • Fonction : Auteur
Liang Zhang
  • Fonction : Auteur
Alexandre Pillon
  • Fonction : Auteur
Mireille Haon
  • Fonction : Auteur
Sacha Grisel
  • Fonction : Auteur
David Navarro
  • Fonction : Auteur
Ian Black
  • Fonction : Auteur
Virginie Neugnot
  • Fonction : Auteur
Parastoo Azadi
  • Fonction : Auteur
Breeanna Urbanowicz
  • Fonction : Auteur

Résumé

Pectin is a complex plant heteropolysaccharide whose structure and function differ depending on its source. In animal feed, breaking down pectin is essential, as its presence increases feed viscosity and reduces nutrient absorption. Soybean meal, a protein-rich poultry feed ingredient, contains significant amounts of pectin, the structure of which remains unclear. Consequently, the enzyme activities required to degrade soybean meal pectin and how they interact are still open questions. In this study, we produced 15 recombinant fungal carbohydrate-active enzymes (CAZymes) identified from fungal secretomes acting on pectin. After observing that these enzymes were not active on soybean meal pectin when used alone, we developed a semi-miniaturized method to evaluate their effect as multiactivity cocktails. We designed and tested 12 enzyme pools, containing up to 15 different CAZymes, using several hydrolysis markers. Thanks to our multiactivity enzymatic approach combined with a Pearson correlation matrix, we identified 10 fungal CAZymes efficient on soybean meal pectin, 9 of which originate from Talaromyces versatilis. Based on enzyme specificity and linkage analysis, we propose a structural model for soybean meal pectin. Our findings underscore the importance of combining CAZymes to improve the degradation of agricultural co-products.

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Dates et versions

hal-04882190 , version 1 (13-01-2025)

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Lauriane Plouhinec, Liang Zhang, Alexandre Pillon, Mireille Haon, Sacha Grisel, et al.. Unlocking soybean meal pectin recalcitrance using a multi-enzyme cocktail approach. Scientific Reports, 2025, 15 (1716 (2025)), ⟨10.1038/s41598-024-83289-4⟩. ⟨hal-04882190⟩
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